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Fig. 2. Autofluorescence of Calliphora rhabdomeres. Confocal images of a frontal region of the intact Calliphora compound eye after simultaneous excitation with 476 nm and 488 nm wavelength laser light show (A) green fluorescence of R7y rhabdomeres and (B) red fluorescence of R1–6 rhabdomeres. (C) Overlay of A and B. A 20x/0.50 water immersion objective was used on a Leica TCS-SP confocal microscope. Scale bar, 20 µm. (D) Ratio of R7p to R7y rhabdomeres. Mean values were determined by evaluating the fluorescence pattern of randomly chosen regions comprising between 55 and 136 ommatidia from 20 Calliphora compound eyes. Bars are ± S.D.





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