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Files in this Data Supplement:
Fig.·S1. Anti-DH31-R1 and anti-DH44-R1 antisera demonstrate specific staining of transfected HEK-293 cells. HEK-293 cells were transfected with CG8422 (A,B) or with CG17415 (C,D) cDNAs, and then stained with anti-CG8422 (DH44-R1) antibody (A,C) and anti-CG17415 (DH31-R1) antibody (B,D). HEK cells were fixed for 60·min at room temperature in 4% paraformaldehyde in PBS, containing 7% picric acid (v/v). The cells were washed and stained for 1·h at room temperature in primary antibodies at 1:1000; cells were then washed and stained with Cy3-conjugated anti-rabbit antibodies (Jackson Labs) at 1:1000.
Fig.·S2. Summary diagram of antibody staining for the neuropeptides DH31 (red) and DH44 (blue).
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