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Fig. 12. Immunocytochemistry for phosphorylated focal adhesion kinase (pFAK) (primary: rabbit anti-phosphorylated human FAK; secondary: goat anti-rabbit Oregon Green 488) and for NKCC (primary: T4 mouse anti-hNKCC; secondary: goat anti-mouse Alexa Fluor 594). (A) pFAK immunofluorescence was present in all mitochondria-rich cells (green). (B) NKCC immunofluorescence (red) had a similar distribution to pFAK. (C) There was a high degree of colocalization of NKCC and phosphorylated pFAK (yellow). (D) Genistein pretreatment (100 µmol l-1, for 1 h) to inhibit tyrosine kinase before addition of the primary anti-pFAK antibody eliminated pFAK immunofluorescence. Bar, 20 µm.





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