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Fig. 2. (A) The expression of Na+,K+-ATPase (NKA) mRNA in the gill was determined using the antisense probe and was found in distinct cells on the primary filament and lamellae (large arrowheads). (B) The sense Na+,K+-ATPase mRNA probe revealed no staining. (C,E) The Na+,K+-ATPase mRNA and protein were colocalized within the same cells (small arrows). (D,F) Double labeling for Na+,K+-ATPase mRNA and nNOS revealed colocalization of these enzymes (arrowheads). Scale bar, 100 µm.





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