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Fig. 4. (A) Proportion of caterpillars that froze at each exposure to –5.5°C. Filled squares, broken line: treatment animals, exposed to –5.5°C each time; N=43. Open triangles, solid line: handling controls, exposed to –5.5°C for the first time at each exposure; N=16. (B) Least-squares mean temperature of crystallisation (Tc) ± 95% confidence intervals (after ANCOVA with body mass as covariate) of control (open triangles, solid line) and treatment (filled squares, broken line) caterpillars exposed to repeated cold events. Only individuals that froze every time are included in the treatment data. Control animals were exposed to the same handling as the treatments, but each exposure was their first exposure to cold for those individuals.





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