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Fig. 1. Diagram of the epi-microspectrophotometric apparatus. The monochromatic beam contains: Qm, Ealing 150 W optically stabilized Xenon-arc illuminator; Fs, heat filter and stray light filter; G, computer-controlled monochromator with entrance slit An and exit slit Ax; Lm, quartz condenser; Af, field aperture; W, computer-controlled counter-rotating neutral density wheels; Sm, computer-controlled Uniblitz shutter; M, mirror. The adapting beam contains: Qa, 45 Wquartz-iodine illuminator; Fh, heat filter; Fd, neutral filter; Fc, cut-off filter; La, telephoto lens; Aa, field aperture; Sa, Uniblitz shutter. The two collimated beams are combined in quartz beam-splitter Bq and inserted into the Leitz Pol-Opak epi-illuminator equipped with 620-257 illumination slide designed for low stray-light. A, aperture diaphragm; Lo, microscope objective, either Leitz 8X/0.18P acromat/215 or Zeiss Luminar-25 mm/0.15; Le, GF10X eye piece; E, eye of completely intact butterfly mounted on Leitz UT-4 goniometric stage. The Leitz UV-MPV photometer head contains Ap, adjustable/centrable photometer diaphragm; Fp, stimulus-blocking filter; P, cooled Hammamatsu R928 photomultiplier.





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