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Fig. 1. S. domuncula arginine kinase. (A) The sponge deduced protein
(AK_SUBDO) is aligned with the arginine kinase from the Cnidarian
Anthopleura japonica (KARG_ANTJA, O15992;
Suzuki et al., 1997), and
Drosophila melanogaster (AK_DROME, AAA68172, glycocyamine kinase
from Nereis virens (GLYCAM_NEREIS, AAL26699 and human creatine
kinase (CK_HUMAN, AAC31758 Mariman et
al., 1987). Residues conserved (similar or related with respect to
their physico-chemical properties) in all sequences are shown in white on
black and those in at least three sequences in black on gray. The two
characteristic domains for the ATP:guanido phosphotransferases, the N-terminal
domain (ATP_GUA_N) and the C-terminal catalytic domain (ATP_GUA_C), are
marked. The A. japonica arginine kinase is a two-domain enzyme, so
the two phosphotransferase domains are also marked for this protein. The
residues involved in coordinating Mg2+ to ATP are marked [ATP]. (B)
Radial, unrooted phylogenetic tree, which includes the above mentioned
sequences; the two domain enzyme from A. japonica has been split into
the N-terminal and C-terminal segments (KARGn_ANTJA, aa1 to
aa367; KARGc_ANTJA, aa368 to aa715). Shown in
addition are the deuterostomian related sequence from (1) the Holothuria
Stichopus japonicus (AK_STICJA, BAA76385, (2) the insect
Schistocerca americana (AK_SCHIAM, AAC478301), (3) the mollusks
Solen strictus (Bivalvia) (AK_SOLSTRI, BAB91358 N terminus
aa1 to aa372 and C terminus aa373 to
aa724; Suzuki et al.,
2002), Corbicula japonica (Bivalvia) (AK_CORBJA,
BAB913571; N terminus aa1 to aa372 and C terminus
aa373 to aa723;
Suzuki et al., 2002) and
Ensis directus (Bivalvia) (AK_ENSIS, AAM906981; N terminus
aa1 to aa372 and C terminus aa373 to
aa723; Compaan and Ellington,
2003), (4) the oyster Crassostrea gigas (Bivalvia)
(AK_CRASGI, BAD119501), (5) Crustaceans Homarus gammarus
(KARG_HOMGA, 538542), Eriocheir sinensis (AK_ERISI, AAF43437 and
Artemia franciscana (KARG_ARTSF, Q95V58), and (6) the nematode
Caenorhabditis elegans (AK_CAEEL, NP_492714.1). Gene duplications
within the mollusks and the Cnidarian taxa are indicated (D). The scale bar
indicates an evolutionary distance of 0.1aa substitutions per position in the
sequence.
