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Fig. 5. Quantification of flightin in normal and transgenic IFM. (A) Western blot of 12% SDS-PAGE probed with an anti-flightin specific antibody. Lane 1, fln0; lane 2, molecular weight marker; lane 3, wild type; lane 4, P{fln+}2.2; lane 5, P{fln+}0.2; lane 6, w; P{w+Mhc+}wm2. (B) Relative intensity of the flightin bands in A. (*P<0.05 vs wt, N=3). Each lane was loaded with 0.15 µg of protein.





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