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Fig. 4. Western blot analysis of 2DE reveal normal temporal phosphorylation of
flightin in transgenic line. (A,C,E) wild type; (B,D,F)
P{fln+}0.2. A and B, P15 pupa; C and D, <1 h
post-eclosion; E and F, 2–4-day-old adult. For all blots, the basic end
is to the left. 2D gel analysis was followed by immunoblot analysis using
anti-flightin polyclonal antibody. N1 and N2 are the non-phosphorylated
isoelectic variants while P1 through P9 are phosphorylated variants (see
Vigoreaux and Perry, 1994).
For each of the developmental stages, P{fln+}0.2
transgenic flies have a nearly identical phosphorylation pattern when compared
to the wild type.
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