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Fig. 3. Transepithelial Na+ and Cl- flux measurements before and after apical treatment with 10 µmol l-1 amiloride. (A) Unidirectional, apical-to-basolateral (apical-blm), basolateral-to-apical (blm-apical) and net ((apical-blm)-(blm-apical)) Na+ fluxes across epithelial tissues bathed in symmetric avian saline solution and voltage clamped at 0 mV (N=6). (B) Unidirectional and net Cl- fluxes across under the same conditions stated in A (N=5). (C) Effects of apical amiloride (10 µmol l-1) on Isc and conductance (G) measurements obtained from tissues used in the transepithelial flux experiments. *Significantly different from control value (P<0.05).





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