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Fig. 2. Effects of apical Na+ channel blockers on basal Isc. (A) Time course of Isc inhibition following sequential treatment with 10 and 100 µmol l-1 amiloride. (B) Concentration–response relationships for amiloride and benzamil on basal Isc. The data was fitted using a four-parameter logistic function and the IC50 values were 120 nmol l-1 (N=5) and 810 nmol l-1 (N=5) for benzamil and amiloride, respectively. (C) Magnitudes of amiloride-sensitive (10 µmol l-1) Isc in tissues from hens where shell formation was complete (N=6) compared to tissues where the egg had not entered the shell gland (N=5). *Significantly different from control value (P<0.05).





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