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Fig. 4. Voltage dependence of activation of the Na+ currents, determined by I–V relationships for the Na+ currents elicited by the voltage steps. Smooth curves are simple Boltzmann functions. (A) V1/2=–45.7 mV, k=–6.4 mV for perforated recording (filled circles), V1/2=–46.1 mV, k=–5.2 mV for conventional recording (open circles). (B) V1/2=–35.5 mV, k=–7.6 mV for 0.5 mmol l–1 GTP{gamma}S (filled triangles), V1/2=–45.1 mV, k=–4.4 mV for 1 mmol l–1 GDPßS (open triangles). For comparison, the broken line for conventional recording is plotted in B. The values are means ± S.E.M. obtained from 6–18 cells.





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