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Fig. 4. (A) Three-dimensional (3D) reconstruction of the position of the Perspex® grooves of the phantom by tracking the tip of a wand with motion analysis (blue) and embedding the position of the grooves when visualised by an ultrasound probe into the laboratory frame of reference (red). The line represents a 3D regression line placed through the motion analysis data for each groove. The inter-quartile range (IQR) of the perpendicular distance of the motion analysis measured coordinates along the Perspex® grooves from the regression line in both 2D (x–z plane) and the 3D were 0.44 mm and 2.12 mm, respectively. (B) Two-dimensional (2D) comparison of the position of the grooves as determined by regression through the motion analysis data and the position determined by the ultrasound technique for each Perspex® plate. This was measured in the same plane (x–z) that was used to image the muscle–tendon junction during the hopping experiments. (C) Histogram of the error of the ultrasound technique compared to the calculated regression line that represents the position of the phantom grooves in both 2D and 3D. The IQR of the perpendicular distance from the measured coordinates of the grooves measured with the ultrasound technique to the linear regression line in both 2D (x–z plane) and 3D were 0.98 mm and 1.15 mm, respectively.





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