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Fig. 6. Changes in Na⁺, Ca²⁺ and K⁺ currents during 10 repetitive depolarising pulses with a 200 ms interval. (A) Upper panel: pulse protocol of the repetitive stimulations. Step depolarisations from the holding potential of –70 mV to +30 mV. Lower panel: superposition of 10 traces of Na⁺ currents in response to step depolarisations. The bath solution was N-Ca-free-ASW, and the pipette solution was Cs-asp. Cell membrane capacitance (C_m)=68.0 pF. (B) Superposition of 10 traces of Ca²⁺ currents. The pulse duration was 25 ms. The bath solution was 4Ca-ASW containing 4 µmol l^–1 TTX and the pipette solution was Cs-asp. C_m=64.9 pF. (C) Superposition of 10 traces of K⁺ currents. The pulse duration was 25 ms. Note that K⁺ current was observed only during the first pulse. The bath solution was N-Ca-free-ASW containing 4 µmol l^–1 TTX, and the pipette solution was K-asp. C_m=187.9 pF.

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