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Fig. 7. Detection of phosphorylation in (A) demembranated and (B) glycerol-treated sperm treated with various solutions. 32P-labelled proteins were separated by a 12.5% separating gel. Demembranated sperm were reactivated in the presence of cAMP. Glycerol-treated sperm showed motility in the 100 mmol l–1 NaCl and KCl solutions. Bars on the left side show molecular mass standards.





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