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Fig. 5. Effect of inhibitors on glycerol-treated sperm. Sperm were incubated for 30 min in ASP containing (A) CCCP, an uncoupler of the mitochondrial electron transport chain for ATP synthesis; (B) SQ22536, an adenylyl cyclase inhibitor; (C) H-89, a PKA inhibitor. Gray bars, percentage motility of intact sperm; black bars, glycerol-treated sperm. Motility was determined from video recordings taken 5 s after motility initiation. Values are means ± S.D. (N=3).





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