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Fig. 6. Induction of Hsp70 expression by thermal stress and/or 1 µmol
l1 CuCl2 in M. galloprovincialis gill
tissue. (A) Hsp70 was detected in extracts (100 µg of protein) from gill
tissue of animals incubated at 30°C for 30 min either in the absence or
presence of 1 µmol l1 CuCl2 or at 15°C
with 1 µmol l1 CuCl2 in the absence or
presence of 1 µmol l1 SB203580 for 30 min. (B)
Densitometric analysis of Hsp70 bands by laser scanning. Results are means
± S.E.M. for four independent
experiments performed with similar results. The molecular mass markers (kDa)
are shown to the right of the panel.
P<0.001,
**P<0.01 versus control value.