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Fig. 5. DNA fragmentation and caspase-3 activation in the mantle and gill tissues
from M. galloprovincialis specimens treated with various heavy
metals. (A) (Left panel) DNA fragmentation induced by 1 µmol
l–1 CuCl2 for 30 min, in the absence or presence
of 1 µmol l–1 SB203580, or 50 µmol l–1
ZnCl2 for 30 min in the mantle tissue. (Right panel) DNA
fragmentation induced by 1 µmol l–1 CuCl2 for
30 min, 50 µmol l–1 ZnCl2 for 30 min or 1
µmol l–1 CdCl2 for 60 min in the gill tissue.
Gels shown are representative of three independent experiments performed with
similar results. (B) Specimens (four animals per group) were incubated in
normal seawater (controls) or treated with either 1 µmol
l–1 CuCl2 in the absence or presence of 1 µmol
l–1 SB203580, 50 µmol l–1
ZnCl2 or 1 µmol l–1 CdCl2 (for 30,
30 or 60 min, for each heavy metal, respectively). Endogenous full-length
pro-caspase-3 and large active fragments of caspase-3 were detected using a
specific rabbit monoclonal antibody in extracts (100 µg of protein) from
mantle (top panel) and gill (bottom panel) tissues. Western blots shown are
representative of four independent experiments performed with similar results.
n.s., non specific.
