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Fig. 2. Effect of SB203580 on the mantle tissue p38-MAPK activation by 1 µmol
l–1 CuCl2. SB203580 (1 µmol
l–1) was added to normal seawater and, after a 15 min
equilibration period of the animals, it was present throughout the experiment.
Phosphorylated (A, top panel) and total (A, bottom panel) p38-MAPK levels were
assayed in mantle tissue extracts (100 µg of protein) from control animals,
as well as from animals treated with 1 µmol l–1
CuCl2 in the absence or presence of the inhibitor. Western blots
shown are representative of four to six independent experiments performed with
similar findings. The molecular mass markers (kDa) are shown to the right of
the panel. (B) Densitometric analysis of phospho-p38-MAPK bands by laser
scanning. Results are means ± S.E.M.
for four independent experiments. 
P<0.001
versus control value.
