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Fig. 5. Transepithelial flux of Texas Red as a function of bathing saline Texas Red
concentration for Drosophila tubules set up in Ramsay assays. Each
point shows the mean ± S.E.M. for
N=730 tubules. The solid line represents the fit to the
MichaelisMenten equation by non-linear regression analysis. The inset
shows the ratio of the concentration of Texas Red in the secreted fluid to
that in the bathing medium (S/M) as a function of bathing medium Texas Red
concentration. As discussed in the text, the point at 50 µmol
l1 Texas Red was excluded from the curve-fitting
procedure.