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Fig. 4. Secreted fluid Texas Red concentration ([Texas Red]sf) as a function of bathing saline Texas Red concentration for cricket tubules set up in Ramsay assays. Each point shows the mean ± S.E.M. for N=6–13 tubules. The solid line represents the fit to the Michaelis–Menten equation by non-linear regression analysis. As discussed in the text, the point at 50 µmol l–1 Texas Red was excluded from the curve-fitting procedure. The inset shows the ratio of the concentration of Texas Red in the secreted fluid to that in the bathing medium (S/M) as a function of bathing medium Texas Red concentration.





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