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Fig. 1. CLSM images showing accumulation of Texas Red in the lumen and/or cells of
Malpighian tubules isolated from crickets (A–D), adult
Drosophila (E) or larval Drosophila (F). The bathing saline
(B), lumen (L) and cells (C) are indicated in
each image. Tubules were bathed in 10 µmol l–1 Texas Red
for 15–30 min before each image was recorded. Tubule diameter is 
60
µm in A–C and 35 µm in D,E. Panel B shows the fluorescence
intensity profile from the base to the tip of the 110 µm-long arrow in A.
Note the presence of large (C) or small (D) spherical concretions in the lumen
of the cricket tubules. Some of the concretions in D appear to accumulate high
concentrations of Texas Red and appear white whereas others are dark or
opaque. The presence of large numbers of small intracellular concretions
prevents clear distinction of luminal from cellular compartments in
Drosophila tubules (E,F).
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