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Fig. 5. RT-PCR to amplify chicken renal OAT1-like, OAT3-like, MRP2-like and
MRP4-like cDNA. mRNA isolated from chick PTCs was reverse transcribed, and the
resulting cDNA amplified using primers to chicken OAT1, OAT3, MRP2 and MRP4
(see Table 1). The RT-PCR
products were run on a 2% agarose gel and stained with Gel-Star. The cDNA
template was either present (+) or absent (–). No product was obtained
when the cDNA template was omitted. Molecular size (base pairs) is indicated
on the left.
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