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Fig. 4. Structure of the SALMFamide precursor gene in Strongylocentrotus
purpuratus. The DNA sequence of the gene is shown in lowercase letters
with base positions in contig 347664 shown in the column on the right
(11762-13920). The predicted precursor sequence is shown in uppercase letters
with amino acid positions shown in bold and in brackets in the column on the
right (1-266). The gene comprises two exons, whose positions are indicated by
the underlying amino acid sequence in uppercase letters, with the start and
end of the intervening intron labelled in bold letters (gt/ag). 24 bases
upstream of the start codon (atg) in the first exon is a putative
TATA-box-like promoter sequence (tttatt), which is shown in bold. The
predicted signal peptide sequence encoded by the first exon is shown in
underlined italics, based on the analysis shown in
Fig. 3B. Amino acid sequences
corresponding to putative SALMFamide neuropeptides encoded by the second exon
are shown underlined, with monobasic and dibasic (K, KR, RR) cleavage sites
shown in bold. In the 3' region of the gene there is consensus
polyadenylation signal sequence (aataaa) located 47 bases downstream from the
stop codon (*).
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