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Fig. 3. Identification of a SALMFamide precusor gene in Strongylocentrotus
purpuratus. (A) Output data from a tBLASTn analysis of
Strongylocentrotus purpuratus genomic sequence data using a putative
starfish SALMFamide-1 (S1) precursor sequence (GFNSALMFGKR x 6) as the
query. A region of contig 347664 was identified as the sea urchin sequence
with the highest level of sequence identity/similarity with the query sequence
(E=8e-04). The translated sequence of bases 13566-13718 from contig 347664 is
shown aligned with the query sequence and four putative sea urchin SALMFamide
neuropeptides are underlined. (B) Diagram showing data obtained from SignalP
3.0 analysis of a 53 amino acid residue sequence encoded by bases 11827-11986
of contig 347664, using hidden Markov models based on eukaryotic data for
signal peptide prediction. The red line shows cleavage site probability,
indicating that cleavage occurs between residues 25 (S) and 26 (F). The green,
dark blue and light blue lines indicate that the first 25 residues of the
sequence conform with high probability to n-terminal (n), hydrophobic (h) and
c-terminal (c) regions, respectively, that are characteristic of eukaryotic
signal peptides. Collectively, these data provide strong evidence that the
first 25 amino acids of the 53 residue sequence are likely to function as a
signal peptide, which is a characteristic feature of all neuropeptide
precursors.
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