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Fig. 1. SALMFamide-like immunoreactivity in whole-body extracts of Echinus
esculentus. The graphs show SALMFamide-like immunoreactivity in a 40%
ACN/TFA Sep-Pak eluate (A,B) and a 60% ACN/TFA eluate (C,D) after HPLC
fractionation using a gradient of ACN/TFA indicated by the line. HPLC
fractions were radioimmunoassayed for both S1-(A,C) and S2-(B,D)
immunoreactivity using the antisera SLII and BGI, respectively. The 40%
Sep-Pak eluate contains two S2-immunoreactive peaks, 1 and 2 (B), which are
weakly immunoreactive with the S1 antiserum SLII. The 60% Sep-Pak eluate also
contains two S2-immunoreactive peaks, 3 and 4 (D), which are weakly
immunoreactive with the S1 antiserum SLII. The elution times of peaks 2 and 3
are the same so they may contain identical immunoreactive peptides. The SLII
antiserum also detects a few minor immunoreactive peaks, which elute before or
after the major peaks 1, 2, 3 and 4.