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Fig. 2. The effects of L-Arg and L-NAME supplementation and
different temperatures (±1°C) on the iNOS protein level in IBAT.
(A) Western blotting was performed using specific antibody against iNOS. The
results of three observations from a representative experiment and the
position of molecular mass markers (kDa) are shown. C, control. (B) Data
obtained after quantification of iNOS bands by ImageQuant software. iNOS
concentration is expressed in relative to the levels in controls acclimated to
room temperature (taken as 100%). The values represent the means ±
S.E.M. from three independent experiments. *Comparison
of the same treatments at different temperatures,
**P<0.025; ***P<0.005;
comparison of different treatments with the control kept at
the same temperature, P<0.025;
P<0.005. Volume is the sum of
all the pixel intensities within a band; 1 pixel=0.007744 mm2.
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