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Fig. 10. Developmental profile of msslo expression in the thoracic dorsal
longitudinal muscles (DLM). Small fluctuations in msslo mRNA levels
occur during the transition from the feeding (V) to wandering (W) larval
stages and from W to pupal ecdysis (P0). Transcript expression remains low in
the early pupal stages (P1-P6), with a moderate increase occurring at pupal
days 8-10. The massive upregulation of msslo expression occurs late
in adult development on day 15 and appears to gradually decline 2 days after
the adult emerges. Total RNA (1 µg per lane) was isolated from thoracic
DLMs from days 0 and 2 fifth instar larvae (V0, V2), days 0, 2 and 3 of
wandering larvae (W0, W2, W3), and days 0, 2, 4, 6, 8, 10, 12, 14 and 15 after
pupation (P0, P2, P4, P6, P8, P10, P12, P14, P15), pharate adults (PA) and in
adults just after they emerged and 2 days after emergence (A0, A2). The RNA
was hybridized to the digoxigenin-labeled msslo 680 bp cDNA insert or
loading control M. sexta ribosomal protein S3 (rpS3) cDNA insert
(Jiang et al., 1996). The
sizes (kb) of the hybridizing RNAs are shown on the left.
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