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Fig. 3. Retinoid cycles during light and dark adaptation in young chickens. Chicken were dark adapted overnight before subjected to light exposure (2000 lux for duration indicated in the figure). Retina was dissected free of the retinal pigment epithelium (RPE), and retinoids were extracted (see Materials and methods) and then analyzed by HPLC. (A) 11-cis retinal (broken line) and 11-cis retinyl ester (solid line) pools during light (open bar) and dark (shaded bar) adaptation (note different scales for retinal and retinyl ester on y1- and y2-axes). (B) All-trans retinal (broken line) and all-trans retinyl ester (solid line) pools during light (open bar) and dark (shaded bar) adaptation. Means ± S.E.M.. were calculated from the results of three experiments. For each of the three experiments, retinoid extracts from individual retina or RPE were analyzed by HPLC and results from two retinae or two RPE (per time point) were averaged (N=2).





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