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Fig. 3. Determination of intracellular pH (pH_i) with intracellular microelectrodes and by application of microfluorometry of SNARF-1-dextran, calibrated with standards containing 100 mmol l^-1 phosphate, BSA and 150 mmol l^-1 KCl. Embryonic cells were equilibrated with P_CO2s alternating between 2.4 and 24.6 mmHg (A). Differences of optical measurements as compared with microelectrode readings indicate the best approximation to intracellular conditions to be achieved by addition of 10% BSA + 150 mmol l^-1 KCl (B).

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