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Fig. 9. Enhanced production of reactive oxygen species in G. dibranchiata erythrocytes exposed to sulfide (up to 1.9 mmol l-1) for 1 h. (A) Formation of fluorescent 2',7'-dichlorofluorescein (DCF) from nonfluorescent 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) in erythrocytes (closed circles) or incubation buffer alone (open circles). DCF fluorescence is normalized to DCF formation in erythrocytes under control conditions (no sulfide). (B) Oxidation of MitoSOXTM Red in erythrocytes. Fluorescence is normalized to control conditions (no sulfide). Values are means ± S.D. (N=4). Asterisks represent a significant effect of sulfide compared to the control value (no sulfide) by RM ANOVA (***P<0.005).





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