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Fig. 3. Fluorescence of JC-1 (A,B) and TMRM (C,D) in G. dibranchiata
erythrocytes exposed to sulfide (A,C; 0.11-1.9 mmol l-1) or CCCP
(B,D; 0.10 mmol l-1) for 1 h. JC-1 fluorescence ratio and TMRM
fluorescence emission at each sulfide concentration are normalized to that of
control erythrocytes (erythrocytes incubated in sulfide-free buffer for 1 h).
Values are means ± S.D. (N=8 for JC-1,
N=4 for TMRM). CCCP (0.10 mmol l-1) was added as a
positive control for loss of 
m. The regression line represents a
sigmoidal dose-response curve (see equation in text) with correlation
coefficient, significance and EC50 listed in each panel. Asterisks
represent a significant effect of sulfide or CCCP compared to the control
value (no toxin) by RM ANOVA (*P<0.05,
**P<0.01, ***P<0.005).
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