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Fig. 2. Distribution patterns of ion-transporting proteins in mitochondrion-rich
cells (MRCs) in the yolk-sac membrane of tilapia yolk balls incubated in
freshwater (AD) and seawater (EH). The yolk-sac membranes were
stained with anti-Na+/K+-ATPase (red; A,E),
anti-Na+/K+/2Cl cotransporter (NKCC)
(blue; B,F), and anti-cystic fibrosis transmembrane conductance regulator
(CFTR) (green; C,G). (D,H) Merged images of AC (D) and EG (H).
Arrowheads and arrows indicate apical NKCC staining and apical CFTR staining,
respectively. (I, II, III, IV) Functional classification of MRCs is based on
distribution patterns of Na+/K+-ATPase, NKCC and CFTR,
according to Hiroi et al.
(2005). Scale bar, 10
µm.