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Fig. 2. Distribution patterns of ion-transporting proteins in mitochondrion-rich cells (MRCs) in the yolk-sac membrane of tilapia yolk balls incubated in freshwater (A–D) and seawater (E–H). The yolk-sac membranes were stained with anti-Na+/K+-ATPase (red; A,E), anti-Na+/K+/2Cl cotransporter (NKCC) (blue; B,F), and anti-cystic fibrosis transmembrane conductance regulator (CFTR) (green; C,G). (D,H) Merged images of A–C (D) and E–G (H). Arrowheads and arrows indicate apical NKCC staining and apical CFTR staining, respectively. (I, II, III, IV) Functional classification of MRCs is based on distribution patterns of Na+/K+-ATPase, NKCC and CFTR, according to Hiroi et al. (2005). Scale bar, 10 µm.





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