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Fig. 2. (A) Identification of troponin T (TnT) S1 and S2 isoforms in rainbow trout using western blotting. Lane 1 is a pre-stained ladder of protein standards (BioRad), and lane 2 is authentic mammalian troponin used as a control (Sigma T-3515). Lane 3 is the 75 mmol l–1 phosphate hydroxy-apatite column fraction that contains two isoforms of purified rainbow trout red muscle TnT, identified as TnT S1 and S2. (B) Determination of sizes of TnT isoforms using a silver-stained PAGE gel. Lane 1 is a protein ladder (BioRad), and lane 2 is the purified TnT.





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