|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Copper accumulation by intestinal cells exposed to external [Cu]
(Cuo) ranging from 0 (no added Cu) to 800 µmol
l–1 for 15 min in normal saline (NaCl=140 mmol
l–1 at pH 7.4, filled triangles), compared with the effects
of either low external chloride (Cl–o=6.6 mmol
l–1, Cl–o replaced by sodium
gluconate, filled circles), 0.1 mmol l–1 DIDS in normal
saline (open squares), or with low external pH (pH 5.5, open circle). Values
are means ± S.E.M. of N=5–6 separate
experiments using fresh cells from different individual fish. Rapid Cu
accumulation in/on cells at time zero was not deducted from the data.
Different letters (a, b, c or d) indicate a statistically significant
difference between adjacent treatments within Cuo (looking up at
adjacent data points between plots at each Cuo,
Kruskal–Wallis test, P<0.05). For clarity, statistical
differences between treatment effects at 10 µmol l–1
Cuo are not shown, but are identical to those at 50 µmol
l–1 Cuo. Cu accumulation within treatment (Cu-dose
effects within each experiment) were significantly different from the control
with no added Cu at Cuo=50 µmol l–1 or greater
for the low Cl–o and low pH experiments; and at 10
µmol l–1 Cuo or greater for the DIDS experiment
(labels not added for clarity, Kruskal–Wallis test, P<0.05).
The effect of DIDS reached a plateau at 200 µmol l–1
Cuo because Cu accumulation rates between 200–800 µmol
l–1 Cuo were not statistically different from each
other within DIDS treatment (from post-hoc box whisker plots
following Kruskal–Wallis test). Similarly, the low pH effect reached a
plateau at 400 µmol l–1 Cuo because Cu
accumulation rates between 400–800 µmol l–1
Cuo were not statistically different from each other within the low
pH treatment (from post-hoc box whisker plots following
Kruskal–Wallis test). The above statistics for Cu-dose effect within
treatment are not shown for clarity.
|
