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Fig. 10. Na+ (white bars) and K+ content (grey bars) content of intestinal cells exposed external [Cu] (Cuo) ranging from 0 (no added Cu) to 800 µmol l–1 for 15 min (A) in low external chloride (Clo=6.6 mmol l–1, Clo replaced by sodium gluconate), (B) with 0.1 mmol l–1 DIDS in normal saline, and (C) at low external pH (pH 5.5). Values are means ± S.E.M. of N=5–6 separate experiments using fresh cells from different individual fish. *Statistically significant difference from control with no added Cu within treatment and electrolyte (Kruskal–Wallis test, P<0.05).





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