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Fig. 5. V-H+-ATPase in the membrane fraction of gills of sham-operated, NaCl-, acid- and base-infused fish. (A) Representative quantitative immunoblotting against the A-subunit of the V-H+-ATPase: a distinct band of ~77 kDa was obtained. (B) Fluorometric analysis revealed that the abundance of the A-subunit of the V-H+-ATPase in base-infused fish increased to 300±81% of sham-operated fish (100±28%) (N=4). (C) V-H+-ATPase activity increased in concert with the increase in abundance noted in B (N=4). Values are mean ± S.E.M. *P<0.05 (one-way ANOVA, Dunnet's post test).





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