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Fig. 2. Na+/K+-ATPase in the membrane fraction of the gills of sham-operated, NaCl-, acid- and base-infused fish. (A) Representative quantitative immunoblotting against the {alpha}-subunit of the Na+/K+-ATPase: a distinct band of ~102.8 kDa was obtained. (B) Fluorometric analysis revealed that Na+/K+-ATPase abundance in acid-infused fish increased to 315±88% of sham-operated fish (100±22%; N=4). (C) Na+/K+-ATPase activity. Values are mean ± S.E.M., N=4. No significant differences were found among treatments. *P<0.05 (one-way ANOVA, Dunnet's post test).





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