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Fig. 4. Response of SOD activity to +7°C increase above ambient. SOD activities
of the three compartment of A. viridis (A) ectoderm, (B) endoderm and
(C) FIZ (freshly isolated zooxanthellae), (D,E) total extract of A.
schmidti under control conditions 0 (17°C) and after 1, 2 and 5 days
of incubation to elevated temperature (24°C), revealed by native PAGE (8%)
and NBT staining, either without KCN (–KCN) or with (+KCN).
Pharmacological identification of the bands is indicated by arrows: MnSOD
(black arrows), CuZnSOD (open arrows) and FeSOD (grey arrows). 150 µg of
protein was loaded in each well. These patterns were reproducible in four
independent experiments for each species.
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