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Fig. 2. Response of SOD activity to experimental hyperoxia. (A) SOD activity of the
three separated compartments of A. viridis (Ec, ectoderm; Ed,
endoderm; FIZ, freshly isolated zooxanthellae). (B) Total extract of A.
schmidti in two conditions: control (C; air saturated) and hyperoxia
(H,; O2-saturated seawater), revealed by native PAGE
(8%) and NBT staining, either without KCN (KCN) or with (+). Arrows
indicate pharmacological identification of bands: MnSOD (black arrows),
CuZnSOD (open arrows) and FeSOD (grey arrows). (d) Corresponds to an
O2-induced SOD isoform (AsSODd). Cytosolic fractions containing a
quantity of 150 µg of protein was loaded in each well. These patterns were
reproducible in four independent experiments for each species.