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Fig. 1. SOD electrophoretic patterns in symbiotic (Anemonia viridis) and non-symbiotic species (Actinia schmidti). (A) SOD activity in A. viridis animal (ectoderm plus endoderm) extract and (B) A. schmidti total extract in the absence (control) and in the presence of KCN (10 mmol l–1) revealed on native PAGE (8%) and by NBT staining. Arrows indicate pharmacological identification of bands: MnSOD (black arrows), CuZnSOD (open arrows). Cytosolic fractions containing a quantity of 150 µg of protein was loaded in each well. Similar results were obtained in four to 10 independent experiments for A. schmidti and A. viridis specimens, respectively.





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