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Fig. 7. Identification of a scolopale at the base of asymmetric setae. Confocal micrographs representing collapsed stacks of optical sections (green, autofluorescence of cuticle; red, phalloidin–AlexaFluor568). (A) Asymmetric seta of a spiny lobster several days after molting as seen in the transmitted light channel. Proximal kink in the shaft (arrow); distal kink in the shaft (double-arrow). (B) The same asymmetric seta as seen in the fluorescence channels. Cuticle autofluorescence clearly delineates the shaft and the socket of the asymmetric seta. Below the socket a phalloidin-positive tube-like structure, the scolopale, is located (arrowhead). Scale as in A. (C) Base region of the same AS at higher magnification. Note tight socket structure and strand-like substructure of scolopale. (D) Another AS of the same animal. Note the generally identical arrangement and the larger distance of the scolopale (arrowhead) to the socket. (E,F) Base of an AS of an animal 1 day after molting. The scolopale is located within the shaft ca. 20 µm distal to the socket (S). (F) Note strand-like substructure of scolopale. (G,H) Base of an AS on the new cuticle of an animal shortly before molting. The scolopale is located within the shaft ca. 20 µm distal to the socket (S). (H) Note strand-like substructure of scolopale.





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