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Fig. 4. Experiment 3. Direct comparison of the effect of the bilateral selective
removal of the asymmetric setae (AS) and the lateral guard setae (left side)
vs the bilateral selective removal of the aesthetascs (AE) and the
medial guard setae (right side) on the mean wipe rate of the antennules after
stimulation with 3 ml of 0.5 mmol l–1 L-glutamate.
Wipe rate values are means ± S.E.M. of N=6
experimental animals (Panulirus argus) in each experimental group
over the 9 trials of an experimental 3-day block; experimental days are
numbered chronologically. Note that selective removal of the AS (Days
08–10, left) is followed by an almost total loss of antennular wiping,
whereas selective removal of the AE (Days 08–10, right) causes no
decrease in the mean wipe rate compared to the mean wipe rate measured in the
unoperated animals in the initial 3-day block (Days 01–03). Pair-wise
comparisons by paired t-tests between the means of the 3-day blocks
reveal a significant difference between the pre-AS-ablation (Days 01–03,
left) and the post-AS-ablation (Days 08–10, left) blocks
(P<0.01), but not between the pre-AE-ablation block (Days
01–03, right) and the post-AE-ablation block (Days 08–10, right)
(P=0.82). Comparison between the post-AS-ablation and the
post-AE-ablation block by an unpaired t-tests reveals a significant
difference between them (P<0.0001). Wipe rates of all 3-day blocks
(means ± S.E.M.): Days 01-03 AS-ablation, 17.7±4.1;
Days 08–10 AS-ablation, 0.7±0.2; Days 01–03 AE-ablation,
16.8±3.0; Days 08-10 AE-ablation, 17.6±2.3.
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