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Fig. 3. (A) Haematocrit (%), (B) plasma osmolality (mOsm kg1) and
(C) plasma angiotensin concentrations (pmol l1) of river
lampreys acclimated to freshwater at 15 mOsm kg1. Control
lampreys (non-injected; black bars) were held under light anaesthesia for 15
min (N=8) and 30 min (N=4) in the absence of further
manipulation. Experimental lampreys were blood sampled at either 15 min or 30
min after an i.p. injection of 1% body mass with either isosmotic saline
(white bars; 120 mmol l1 NaCl; 233 mOsm
kg1; N=5 at each time point) or hyperosmotic saline
(cross-hatched bars; 4 mol l1 NaCl; N=5 at 30 min;
N=8 at 30 min). Different letters above error bars signify groups
that differ significantly (ANOVA and post-hoc multiple comparison
tests).