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Fig. 2. Blood volume depletion in river lampreys acclimated to Kerteminde seawater at 576 mOsm kg–1 (21 p.p.t.). The first blood sample (pre-volume depletion; open bars) was taken to achieve a 40% decrease in blood volume and a second sample (post-volume depletion; hatched bars) was collected after 30 min (N=10), 60 min (N=11) or 90 min (N=7). Data for (A) haematocrit (%), (B) plasma osmolality (mOsm kg–1) and (C) plasma angiotensin concentrations (pmol l–1) are shown (**P<0.01; ***P<0.001, paired t-tests).





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