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Fig. 1. Representative original data recordings illustrating the negligible effects of gas transfer across the air–water interface during respirometry experiments and the contribution of non-piscine metabolism to aquatic O2 and CO2 changes. (A) The effects of incremental increases in aerial PCO2 (grey line) on aquatic PCO2 (black line) during ~35 min of simulated respirometry. (B) The changes in aerial PCO2 (grey line) and aquatic PCO2 (black line) immediately before and after euthanizing (denoted by the broken line) a fish during a respirometry experiment. (C) The PCO2 of the air chamber was lowered after euthanasia by flushing the chamber with air. The changes in aerial PO2 (grey line) and aquatic PO2 (black line) immediately before and after euthanizing (denoted by the broken line) a fish during a respirometry experiment.





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