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Fig. 10. Transport of fluorescent glibenclamide. Tubules were dissected and
incubated in medium containing Texas Red (TR)-labelled glibenclamide. (A-D)
Images taken from a time series at 1 min, 15 min, 30 min and 45 min,
respectively. Panels E-L show features of glibenclamide transport in specific
areas of the tubule: (E) urethra; (F) surface of the main segment, showing the
stellate cells; (G) main segment; (H) initial segment of anterior tubule.
Images A-D were taken 40 min after the addition of 0.1 mmol l-1
TR-glibenclamide. Images I-L were taken of the same regions of the tubule as
E-H but were not exposed to TR-glibenclamide. The same detector gain (0.01)
and off-set amplification (500) were used for all images. Tubule diameters can
be taken as 35 µm throughout.
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