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Fig. 4. Sections at anus level of wild-type larvae aged (A) 48 hpf, (B) 72 hpf and
(C) 96 hpf, and nicb107 larvae aged (D) 48 hpf, (E) 72 hpf
and (F) 96 hpf stained for propidium iodide with muscle fibre trajectories
superimposed. Trajectories are visualised by a white circle for the anterior
starting position and a line segment that represents the direction and
projected distance travelled over the next 5 µm. Azimuth and elevation can
be inferred from the components in the X and Y direction of
the line segment (see main text). All sections are viewed from the anterior
side, and the projections are of fibres running from anterior to posterior
(below the surface of the figure) over a distance of 5 µm. This implies
that the left side of the embryos is to the right and vice versa in
this view. Neural tube (nt) and notochord (nc) are indicated. Scale bars, 20
µm.
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