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Fig. 1. Scheme of the perfusion set-up. The isolated heart was mounted into a
saline-filled, temperature-controlled chamber. The coronary cannula was
connected to two input reservoirs, one filled with saline (maintained at
constant level by a re-circulating pump; not shown) and the other filled with
the red blood cell (RBC) suspension. Perfusion could be shifted between the
two reservoirs via a three-way tap. Each reservoir was associated
with a coronary flow measurement device similar to that described by Agnisola
et al. (1994), but computer
controlled. The aortic cannula was occluded so the output from the preparation
was into the chamber via the atrial cannula. An ISO-NOP electrode was
inserted into the atrial cannula to measure relative changes in NO level in
the effluent from the preparation. The saline level in the chamber was kept
constant by an overflow. Samples could be taken from both input and
atrium.
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