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Fig. 4. Quantitative PCR analysis of mRNA transcript abundance in anterior (3, 4, 5) and posterior (6, 7, 8) gills of Chasmagnathus granulatus following transfer from 30{per thousand} salinity to 2{per thousand}, normalized to the mean of posterior gills from crabs acclimated to 30{per thousand} seawater (zero-time value). (A) Arginine kinase; (B) V-type H+-ATPase B-subunit; (C) Na+/K+/2Cl- cotransporter; (D) Na+/K+-ATPase {alpha}-subunit. Means and standard errors were calculated from gills 3, 4 and 5 (anterior) and gills 6, 7 and 8 (posterior), with triplicate determinations for each gill preparation. Results of statistical analysis by ANOVA and post hoc comparisons are presented in the text.





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